Montmorillonitin Toksik Gıdalara Karşı Koruyucu Olarak Kullanımı
2012 tarihli bir bilimsel çalışmada
aktive
edilmiş Karbon ve Mısır Montmorillonit Kilinin, Deoksinivalenol zehrine
karşı koruyucu olarak kullanımı incelenmiş ve bu çalışmanın sonucunda,
Biochemical Studies On The Protective
Effects Of Egyptian Montmorillonite Clay And Activated Carbon Against
Health Hazards Resulting From The Exposure To Deoxynivalenol Mycotoxin
In Food
Mohamed M. Sief1*, Mona M. Abdel-Galil1,
Farouk A. Khalil2, Abdel-Hady F. Hussein
Food Toxicology & Contaminants Department, National Research Center,
Dokki, Cairo, Egypt, Department of Animal Physiology, Fac. of Agriculture,
Ain-Shams University, Cairo, Egypt Received: 23 Octomber 2012; Accepted:
15 November 2012
Abstract: The current
study aimed to use the Egyptian Montmorillonite clay (EM clay) and Activated
Carbon (AC) derived from dates stones as a protective agent against Deoxynivalenol
toxicity. Forty eight female Sprague-Dawley rats were divided into six
groups including the control group, the group fed basal diet plus EM clay
(0.5%), the group fed basal diet plus AC (0.5) % and other three groups
fed DON- contaminant diet (25 mg / kg diet) alone or plus EM clay (0.5
%) or plus AC (0.5%). At the end of the experimental period, blood samples
were collected for serum biochemical analyses. Animals were scarified
and dissected samples of liver and kidney were collect for histopathology
and determination of antioxidant enzyme activities and lipid peroxidation
in liver. The results indicated that rats fed DON-contaminated diet showed
a significant increase in the activities of ALT, AST, ATP and levels of
uric acid, createnin, total lipids, triglycerides and lipid peroxidation
and also showed significant decrease in Total Antioxidant Capacity (TAC),
Nitric oxide activities and significant decreases in GSH level. Histological
examination of the liver and kidney tissues showed severe histopathological
and histochemical changes. Animals fed DON contaminated diet plus EM clay
or AC showed a significant elimination of the harmful effects of DON in
all biochemical, antioxidant parameters and the histological picture of
liver and kidney.
